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KMID : 1024520170260111285
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Journal of the Environmental Sciences
2017 Volume.26 No. 11 p.1285 ~ p.1295
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Structural Analysis and Biological Activities of Sesquiterpene Lactones Isolated from the Leaves and Stems of Chrysanthemum boreale Makino
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Lee Jong-Rok
Park Moon-Ki
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Abstract
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Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-o-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-o-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an IC50 value of 7 μg/mL, whereas compound 1 did not exhibit significant activity with an IC50 value of over 14 μg/mL against murine macrophage.
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KEYWORD
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FPTase, Nitric oxid, Apoptosis, Sesquiterpene lactone, Chrysanthemum boreale Makino
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